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c trachomatis serovar d  (ATCC)


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    ATCC c trachomatis serovar d
    C Trachomatis Serovar D, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 163 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/c+trachomatis+serovar+d/Chlamydia+trachomatis+Serovar+D/pm41308921-84-3-7
    Average 95 stars, based on 163 article reviews
    c trachomatis serovar d - by Bioz Stars, 2026-10
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    Purification:

    Article Title: Chlamydia trachomatis disrupts host metabolism in primary cervical epithelial cells.
    Article Snippet: .. Propagation and purification of C. trachomatis HeLa-229 cells were infected with C. trachomatis serovar D (ATCC VR-885) as described previously, with minor modifications (Scidmore 2005). .. In brief, confluent monolayers were washed with HBSS, treated with 45 μg/ mL DEAE-dextran (Sigma-Aldrich, MO, USA) for 30 min, and infected by rocking at 37 °C for 3 h. After that, DMEM with 10% FBS, 10 μg/mL gentamicin, and 2 μg/ mL cycloheximide were added, and cells were incubated at 37 °C, 5% CO2 for 48 h. C. trachomatis were purified using density gradient centrifugation.

    Infection:

    Article Title: Chlamydia trachomatis disrupts host metabolism in primary cervical epithelial cells.
    Article Snippet: .. Propagation and purification of C. trachomatis HeLa-229 cells were infected with C. trachomatis serovar D (ATCC VR-885) as described previously, with minor modifications (Scidmore 2005). .. In brief, confluent monolayers were washed with HBSS, treated with 45 μg/ mL DEAE-dextran (Sigma-Aldrich, MO, USA) for 30 min, and infected by rocking at 37 °C for 3 h. After that, DMEM with 10% FBS, 10 μg/mL gentamicin, and 2 μg/ mL cycloheximide were added, and cells were incubated at 37 °C, 5% CO2 for 48 h. C. trachomatis were purified using density gradient centrifugation.

    Article Title: Chlamydia trachomatis disrupts host metabolism in primary cervical epithelial cells
    Article Snippet: .. HeLa-229 cells were infected with C. trachomatis serovar D (ATCC VR-885) as described previously, with minor modifications (Scidmore ). .. In brief, confluent monolayers were washed with HBSS, treated with 45 μg/mL DEAE-dextran (Sigma-Aldrich, MO, USA) for 30 min, and infected by rocking at 37 °C for 3 h. After that, DMEM with 10% FBS, 10 μg/mL gentamicin, and 2 μg/mL cycloheximide were added, and cells were incubated at 37 °C, 5% CO 2 for 48 h. C. trachomatis were purified using density gradient centrifugation.

    Article Title: Haematopoietic innate interleukin 17A production drives immunopathology in female mouse genital Chlamydia muridarum infection.
    Article Snippet: The human fallopian tube epithelial cell line FTE was provided by Ronny Drapkin (Harvard Medical School, USA).31 Cells were grown in DMEM/F12 (no HEPES, no phenol red) supplemented with 2% v/v/ Ultroser/G Serum Substitute (cat: 15950- 017, Thermo Fisher Scientific, Seventeen Mile Rocks, Australia), 1 × penicillin/streptomycin (Thermo Fisher Scientific, cat: 15140148), 1 × Glutamax (Thermo Fisher Scientific, cat: 35050079). .. Cells were infected with C. trachomatis serovar D (ATCC #VR885) at MOI 0.1 in multiple experiments. .. For gene transcript detection via PCR, cell monolayers were scraped into microfuge tubes, and RNA was extracted using the QIAGEN RNeasy RNA extraction kit (cat: 74004). cDNA was generated using the Bio- Rad iScript Reverse Transcription Supermix (cat:1708840) as per the manufacturer's instructions.

    Article Title: Chlamydia trachomatis plasmid-encoding Pgp3 protein induces secretion of distinct inflammatory signatures from HeLa cervical epithelial cells
    Article Snippet: .. Hela-229 cells (ATCC CCL-2.1) were infected with C. trachomatis Serovar D (ATCC VR-885) using a method previously described with minor modifications [ ]. ..

    Article Title: Cervical mucosal inflammation expands functional polymorphonuclear myeloid-derived suppressor cells
    Article Snippet: Cells were then washed with PBS +3% NMS/NGS, and fixed with PBS+1% paraformaldehyde before acquisition on a BD LSR Fortessa flow cytometer (Cytomics Platform, UAT, VHIR). .. HeLa-229 cells (ATCC CCL-2.1) were infected with C. trachomatis Serovar D (ATCC VR-885) as previously described , with minor modifications. ..

    In Vitro:

    Article Title: Sorangicin A Is Active against Chlamydia in Cell Culture, Explanted Fallopian Tubes, and Topical In Vivo Treatment
    Article Snippet: .. C. trachomatis serovar D (ATCC VR-885) and C. muridarum NiggII (ATCC VR-123) were purchased from ATCC and propagated in HeLa (ATCC) or HEp-2 (ATCC) cells in vitro. ..

    Positive Control:

    Article Title: Prevalence of Chlamydia trachomatis, Ureaplasma spp., Mycoplasma hominis and Mycoplasma genitalium in patients with sterile pyuria.
    Article Snippet: .. Template DNA of C. trachomatis serovar D (ATCC VR-885) was used as positive control in each PCR reaction. ..

    Polymerase Chain Reaction:

    Article Title: Prevalence of Chlamydia trachomatis, Ureaplasma spp., Mycoplasma hominis and Mycoplasma genitalium in patients with sterile pyuria.
    Article Snippet: .. Template DNA of C. trachomatis serovar D (ATCC VR-885) was used as positive control in each PCR reaction. ..



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    Groups of female B6C3F1 mice were given a transcervical infection (infection 1, I1) 13 weeks after, the animals were given another infection (infection 2, I2). 7 days after the second infection the animals were taken out for analysis. a Bacterial burden in genital tract (GT) swabs is measured ( n = 12). Bacterial numbers are calculated as log10 of inclusion-forming units (IFU). Line represents median log 10 (IFU) levels with 25th and 75th percentiles. b Overview of the model using antibiotics: The animals were infected at week 0 (W0) with 1.5×10 3 IFU of Chlamydia trachomatis (C.t.) serovar D (SvD). 1, 2 or 3 weeks after the infection, the animals were treated with antibiotics. 13 weeks after the first infection, the mice were given a second infection with 1.5×10 3 IFU of C.t . SvD. c Bacterial levels measured at day 7 post infection no. 2 ( n = 4–12). d , e Total IgG in serum at day 7 post infection 2 shown as titration curve with calculated area under the curve (AUC). f , g , h The effect of week 1 antibiotic treatment on natural immunity 5 weeks post treatment ( n = 8). f Total UV-SvD-specific IgG in serum shown as titration curve g with calculated AUC. Points and error bars indicate means ± SD. h the frequency of cytokine-producing CD44 high(hi) CD4 T cells (cyt+, expressing combinations of TNFα, IL-2, IFNγ and/or IL-17) among all CD4 T cells in GT (D, n = 8) in response to in vitro UV-SvD stimulation (gating strategy Supplementary Fig. ). Bars indicate means ± SD. Statistical significance in ( a ) was evaluated Mann–Whitney test. Statistical significance in ( c ) and ( e ) was determined by Kruskal–Wallis test followed by Dunn’s multiple comparisons test. Unpaired t test was used in ( g , h ) for comparison among groups. * p < 0.05, *** p < 0.001, **** p < 0.0001, ns not significant.

    Journal: NPJ Vaccines

    Article Title: Post-exposure vaccine protection of CTH522/CAF ® 01 against reinfection with Chlamydia trachomatis requires Th1/Th17 but not Th2-immunity

    doi: 10.1038/s41541-025-01117-w

    Figure Lengend Snippet: Groups of female B6C3F1 mice were given a transcervical infection (infection 1, I1) 13 weeks after, the animals were given another infection (infection 2, I2). 7 days after the second infection the animals were taken out for analysis. a Bacterial burden in genital tract (GT) swabs is measured ( n = 12). Bacterial numbers are calculated as log10 of inclusion-forming units (IFU). Line represents median log 10 (IFU) levels with 25th and 75th percentiles. b Overview of the model using antibiotics: The animals were infected at week 0 (W0) with 1.5×10 3 IFU of Chlamydia trachomatis (C.t.) serovar D (SvD). 1, 2 or 3 weeks after the infection, the animals were treated with antibiotics. 13 weeks after the first infection, the mice were given a second infection with 1.5×10 3 IFU of C.t . SvD. c Bacterial levels measured at day 7 post infection no. 2 ( n = 4–12). d , e Total IgG in serum at day 7 post infection 2 shown as titration curve with calculated area under the curve (AUC). f , g , h The effect of week 1 antibiotic treatment on natural immunity 5 weeks post treatment ( n = 8). f Total UV-SvD-specific IgG in serum shown as titration curve g with calculated AUC. Points and error bars indicate means ± SD. h the frequency of cytokine-producing CD44 high(hi) CD4 T cells (cyt+, expressing combinations of TNFα, IL-2, IFNγ and/or IL-17) among all CD4 T cells in GT (D, n = 8) in response to in vitro UV-SvD stimulation (gating strategy Supplementary Fig. ). Bars indicate means ± SD. Statistical significance in ( a ) was evaluated Mann–Whitney test. Statistical significance in ( c ) and ( e ) was determined by Kruskal–Wallis test followed by Dunn’s multiple comparisons test. Unpaired t test was used in ( g , h ) for comparison among groups. * p < 0.05, *** p < 0.001, **** p < 0.0001, ns not significant.

    Article Snippet: Chlamydia trachomatis(C.t) Serovar D (SvD) (ATCC) were grown in HeLa cells (ATCC) in RPMI 1640 media (Invitrogen) supplemented with 1%HEPES, 1% of Non-essential amino acids (NEAA) (MP Biomedicals), 1% L-Glutamin (Gibco) and 1% pyruvate (Gibco).

    Techniques: Infection, Titration, Expressing, In Vitro, MANN-WHITNEY, Comparison